Here, we investigate the ways makeup can communicate ambiguity in a character, and cause a viewer to question their role in the story, and what they mean for the main character.
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Also included are themes of "The Makeover," makeup as a mask or method of fitting in, makeup as a tool, and gender non-conformity.
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Megara wears more makeup than your average heroine, with eyeshadow, stylized brows, and berry lipstick. Her role as the villain's pawn is revealed soon after meeting her, and her job soon becomes seducing the hero and discovering his weakness. She eventually turns out as a good character, but not until closer to the end of the movie.

In pre-production, Elsa was originally intended to be the villain in the movie. Her role is uncertain in the beginning of the movie, as she freezes her home town and runs away. Her makeup is heavier compared to her sister, Anna, with purple eyeshadow, heavy liner, and lipstick.

"Fosters Home for Imaginary Friends," 2004-2009, multiple episodes Duchess is a recurring character and often terrorizes the main characters with her stuck-up attitude. She never does anything nefarious, other than driving everyone nuts with her complaining and demands, but she is one of the only characters in the whole show that wears anything that resembles makeup.

"Atlantis: The Lost Empire," 2001 Helga is a part of the expedition team to find Atlantis and one of four main female characters in the movie. She meets the main character by breaking into his apartment in the middle of a thunderstorm in a provocative black gown and fur coat. She is professional, curt, and very attractive. Helga reveals herself to be on the villain's side later in the movie, but has a hand in defeating him when he turns on her.

Georgette is a prissy show-dog with adoring fans, a room all to herself, a vanity, and eyeshadow. Her song, "Perfect isn't Easy," makes her self-entitlement obvious. She lies to the main character, Oliver, out of jealousy and runs him out of her home. She eventually turns good later in the movie. She's the only character that wears makeup in the movie.

2012-2014, Multiple Episodes Asami is introduced when a male character runs into her with his bike, and promptly falls in love with her. The main character doesn't trust her from the start, put off by her wealth and polished look, but she eventually proves herself as a trustworthy character and a valuable part of the team. Asami has the similar berry lipstick other female ambiguous characters wear.

(2002) Captain Amelia is the stern, no-nonsense captain that guides the main characters to their destination. She buts heads with the main characters early on the movie, but quickly proves herself as a good character. The green eyeshadow, literal cat-eye, and beauty mark add to her character's uncertainty.

2003-2009, Season 1, Episode 6 When Jenny is refused a party invitation and ridiculed for her plain, dirty paint job, she seeks a makeover at a auto shop. They repaint her light blue exoskeleton fire-engine red, add blush, lipstick, and eyelash-shaped windshield wiper. She basks in the glory and attention of her new look, and gets to go to the party when the host sees how pretty she is after the makeover.

1999-Present, Season 5, Episode 19 Squidward is accidentally made handsome when Spongebob slams his face in a swinging door. Squidward enjoys free gifts, special treatment, and female attention because of his new look. He eventually grows "too handsome" and bashes his face on a table to go back to normal. I included a male character that seemingly wears no makeup because it follows the theme of "The Makeover."

2001-2014, multiple episodes Many gadgets are featured on the show disguised as common makeup items and beauty tools. Here, Alex uses a laser lipstick to cut open a ventilation shaft. The use of makeup as a weapon or tool of empowerment is almost never seen, so this positive take on them is refreshing in a children's show.

This gadget underwent a few transformations, going from a simple walkie-talkie device to a recognizance drone and instant transporter.

This is a liquid nitrogen mixture disguised as a perfume. It's often use to freeze henchmen or break out of containment areas.

Other gadgets camera hair clips, translating watches, grappling hook hairdryers, oxygen lipstick, and bracelet tasers.

1962-1963, Multiple episodes Here, Jane Jetson is seen literally putting on her face. She is shown with under-eye lines and messy hair, and puts on a mask-like image of her face.

This image illustrates themes of the makeover and the mask. Mulan is made up to find a husband and bring honor to the family, but it's not who she is, and she wipes it off during her song, "Reflection." Mulan finishes most of the movie posing as a male, and consequentially wears no noticeable makeup.

2001-Present, Season 2, Episode 19 When Timmy wants to get closer to his crush, he wishes for his magical fairies to turn him into a girl. She befriends her crush as a girl and eventually cracks under the pressure of being a female.

Various series from 1930-Present Bugs Bunny often cross dresses to confuse or trick his adversaries. His costumes include breasts, clothing, and makeup. His portrayal as a woman is always for comedic effect, and points at a negative attitude towards gender non-conformity, cross-dressing, and femininity.

1999-Present, Season 3, Episode 53 Spongebob is accused of kissing his boss' Millionth Dollar Bill with "Coral Blue #2 Semi-Gloss Lipstick." He knows the actual shade name of the lipstick, suggesting he wears it often, but gets gut off before he can correct his boss.